WebA. tumefaciens strains EHA 101 and EHA 105 contained the disarmed plasmid pCAMBIA 1304 which contains genes for hygromycin (hyg), β-gluconidase (gusA) and gfp (Fig. 2). The DHα E.coli strain ... WebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β …

Overexpression of the Chitosanase Gene in Fusarium solani via ...

WebNov 25, 2024 · Agrobacterium tumefaciens strain GV3101 was transformed with the pBI121, pCAMBIA 1300, and recombinant pCAMBIA-PTHCA 2-1 Promoter-GUS binary vectors ( Supplementary Figure S1 ). The pBI121 … WebJun 9, 2024 · In the first step, an EcoRI/KpnI fragment of ~ 2.4 kb was excised from the plasmid pKSMYMIV-A and cloned into the EcoRI/KpnI sites of binary vector pCAMBIA-1300 (Cambia) to produce the recombinant plasmid pCAM-MYMIV-AM. the weezy effect 2

pCAMBIA1300 Sequence and Map - SnapGene

WebpCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system. The pCambia1300 vector offers: High copy number in E.coli for high DNA yields WebSep 8, 2014 · The fragment was then ligated with a GFP-containing pCAMBIA 1300 binary vector to develop the vector named as pDsTGFP (Figure 1B). The vector has two AhdI sites at the 13 th and 1329 th position, respectively (Figure 1B). Thus, the 3′ T-overhang ends can be generated by digesting the pDsTGFP vector with the enzyme AhdI (Figure … WebSep 1, 2015 · The pCambia 2300 vector assumes stable genomic insertion and expression in plant species ( Hajdukiewicz et al., 1994) and the proper Gateway®-like function and … the weezy