Cholesterol efflux assay protocol
WebMar 6, 2012 · Cholesterol efflux assay is usually used to assess the influence of natural products on plasma acceptorinduced cholesterol efflux from cells (Low et al., 2012). Dr. Rothblat's group pioneered ... WebJul 8, 2024 · In this manuscript we report the first documented use of the BODIPY-cholesterol assay in spermatozoa, which can successfully quantify cholesterol efflux during in vitro capacitation and has...
Cholesterol efflux assay protocol
Did you know?
WebSummaryAutomatic TranslationMarch 6th, 2012. The cholesterol assay is designed to quantitate the rate of cholesterol efflux from cultured cells and the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of labelling cells with cholesterol, equilibration of cholesterol among intracellular pools and release ... WebMar 22, 2024 · The cholesterol efflux assay showed no difference between HBP treated and untreated with TNFα for 24 h in the presence of HDL and APOA-I ... The resazurin assay was performed based on the protocol of Jennings et al. . A resazurin stock solution at 880 µM (R7017, Sigma) was prepared by dissolving the powder in 0.1 N NaOH (0.011 …
WebDec 1, 2011 · The present study was designed to determine whether a cholesterol efflux assay could be developed that avoided the need to use donor cells that contained radiolabeled cholesterol and eliminated some of the steps needed to establish the efflux capacity of serum, HDL, or apolipoproteins. WebDec 1, 2011 · Studies have shown a negative association between cellular cholesterol efflux and coronary artery disease (CAD). Standard protocol for quantitating cholesterol efflux involves labeling cells with [3 H]cholesterol and measuring release of the labeled sterol.Using [3 H]cholesterol is not ideal for the development of a high-throughput …
WebThe cholesterol efflux assay is designed to quantitate the rate of cholesterol efflux from cultured cells. It measures the capacity of cells to maintain cholesterol efflux and/or the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of the following steps. WebAug 12, 2024 · Cholesterol efflux assay Request a detailed protocol. ... (IACUC) protocols (#07919–2024) of Yale University. At 10 weeks of age, male C57BL/6 J mice (JAX, #000664) were injected with 15 mg/kg lipopolysaccharide (LPS) from E. coli O111:B4 intraperitoneally (Sigma). Six hr later, blood was collected for lipid and cytokine analysis.
WebNational Center for Biotechnology Information
WebBackground: Cholesterol efflux as a key event in reverse cholesterol transport (RCT) is considered now as both diagnostic tool and a promising target for the treatment of atherosclerosis. Radioactive in vitro cholesterol efflux assay (CEA) is the gold standard for determination of efflux at cellular level. Fluorescent tracers and stable isotope-labeled … patara qaltevza 1WebJan 22, 2024 · The protocol for efflux assay used in different laboratories differs by type of cell, acceptor, efflux time, and specificity of the transporters [22, 71, 72]. In order to measure HDL’s ability to take up cholesterol, whole serum, apoB-depleted serum, or isolated HDLs are used as acceptors. apoB-depleted serum is preferred over other HDL ... patara pronounceWebK603-100 is the same size as the 100 test size of ab65359. Other cholesterol assay kits include: - HDL and LDL/VLDL Cholesterol assay kit ab65390. - C ell-based Cholesterol assay kit ab133116. - Cholesterol … ガーミン アクティビティ 検索WebJun 1, 2024 · This latter protocol discards VLDL and LDL and relies upon SE-FPLC to separate Lp(a) and HDL. ... biology including in vitro gene expression assays and measuring cholesterol efflux. patara schiffWebApr 11, 2024 · Two protocols for functional assays were established that show the suitability of this cell line to study both cholesterol uptake and cholesterol efflux. LXR agonists are appropriate positive controls in both assays, leading to decreased cholesterol uptake and to increased cholesterol efflux. ガーミン アプローチ g80 口コミWebCholesterol Assay Kit (Cell-Based) (ab133116) includes filipin III, fixative, and wash buffer in a ready to use format. It provides a simple fluorometric method to study mechanisms and biological factors that regulate cholesterol metabolism or movement within cells. A cholesterol trafficking inhibitor, U-18666A, is included as a positive control. ガーミン アプローチ r10 精度WebSep 1, 2024 · Cholesterol efflux assay. Macrophage-specific efflux capacity was measured using BODIPY-cholesterol by a method reported previously with modifications . J774 mouse macrophages were dispensed into a 48-well plate at 7 × 10 4 cells per well. After 24 h, the cells were incubated for 2.5 h with 8 μmol/L BODIPY-cholesterol and in … pataras 4 lettres