Glut4 antibody cell signaling
WebGlut4 Antibody (IF8) is an IgG 1 κ mouse monoclonal Glut4 antibody (also designated Glucose transporter type 4 antibody, GLUT-4 antibody, or SLC2A4 antibody) suitable … WebGLUT4 Antibody. Check out specifications and order GLUT4 Antibody online. ... 3T3L1 (2), MCF-7 (4) cell lysates and Mouse heart (3) tissue lysate. Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SLC2A4 mouse mAb with DAB staining. ... Western blot was used to determine the levels of insulin signaling related ...
Glut4 antibody cell signaling
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WebMay 16, 2024 · The specific mAbs isolated here recognize native GLUT4 on the cell surface and can distinguish its different conformational states, thus representing some of the only state-specific mAbs ever isolated against any transporter. ... A complex network of signaling pathways and membrane-trafficking events downstream of the insulin receptor … WebSep 1, 2002 · There is a need to understand whether the amount of GLUT4 at the cell surface determines the extent of glucose uptake in response to insulin. Thus, we created a. ... and anti-phospho-ATF-2 (Thr 71) polyclonal antibody from Cell Signaling Technology (Beverly, MA). A polyclonal anti-GLUT4 rabbit antibody was raised in our laboratory to a ...
WebGlucose transporter type 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4, is a protein encoded, in humans, by the SLC2A4 … WebInsulin signaling pathway. Our poster covers glucose interaction with GLUT-4 and the downstream signaling pathways, as well as insulin binding to insulin receptors and the downstream effects of this. Insulin is a peptide hormone synthesized by pancreatic beta cells located in the islets of Langerhans. Secreted in response to elevated levels of ...
Web3T3-L1 prepreadipocytes were cultured in DMEM containing 25 m m glucose, 10% fetal serum at 37 °C, and 5% CO 2.L6 muscle cells expressing c-myc epitope tagged GLUT4 (L6myc cells) were maintained in myoblast monolayer culture in α-MEM containing 10% (v/v) FBS and 1% (v/v) antibiotic-antimycotic solution (100 units/ml penicillin G, 10 μg/ml … WebAug 6, 2024 · GLUT4 and CD36 cell surface delivery is triggered by insulin- and contraction-induced signaling, which share conserved downstream effectors. ... The cells were then incubated with a combination of primary antibody pairs (anti-Rab8a (Rabbit, Cat# 55296-1-AP, Proteintech, Rosemont, IL, USA), anti-Rab11 (Rabbit, Cat# 5589S, Cell …
WebGlut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin … Monoclonal Antibody for studying Glut4. Cited in 185 publications. Validated for …
WebInsulin stimulates glucose transport largely by mediating translocation of the insulin-sensitive glucose transporter (GLUT4) from an intracellular compartment to the plasma membrane. Using single cell microinjection of 3T3-L1 adipocytes, coupled with immunofluorescence detection of GLUT4 proteins, we have determined that inhibition of endogenous p21ras … elevate next facebookWebGlut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation (1,2,4). elevate new orleansWebOct 30, 2001 · The increased cell-surface GLUT4 level in the Rab5 antibody-injected cells could be caused by either a decrease in the rate of the GLUT4 internalization or a delay in GLUT4 internalization, or both. To test specifically the effect of Rab4 and Rab5 on GLUT4 internalization, 3T3-L1 adipocytes were stimulated with 1.7 nM insulin for 20 min. footie sportWebAbcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. ... Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547) at 5 µg/ml + Partial tagged recombinant protein to ... footies pj for womenWebAll lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution Lane 1 : Human fetal heart whole cell lysate Lane 2 : Human fetal heart whole cell lysate with Phosphatase Lysates/proteins at 15 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 … footies shoes in costa mesaWebGlut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles … footies patternWebInsulin Signaling; Warburg Effect; AMPK Signaling ... Western blot analysis of extracts of various cell lines, using GLUT4 antibody at 1:1000 dilution. Secondary antibody: HRP … elevate next new orleans