WebH-2Kb/H-2Db Antibody, anti-mouse, REAfinity™ Recombinant Clone: REA932 Type of antibody: Primary antibodies, Recombinant antibodies Isotype: recombinant human IgG1 Applications: FC, MICS, IF, IHC Applications Specifications Resources References Flow cytometry applications for H-2Kb/H-2Db Antibody, anti-mouse, REAfinity™ APC APC … WebJun 1, 1985 · EL4/Mar is a variant cell line that expresses H-2Db but neither H-2Kb nor beta 2-microglobulin (beta 2m). Southern and RNA blot analysis and immunoprecipitation of metabolically labeled...
Epitope specificity of H-2Kb-restricted, HSV-1-, and HSV-2-cross ...
WebTo investigate the immune response of H-2Db-restricted CD8+ T cells in immunotherapy, we prepared the H-2Db tetramer and verified its effectiveness in enumerating the CD8+ T cells specific for the lymphocytic choriomeningitis virus (LCMV). First, the cDNA encoding H-2Db heavy chain was cloned by RT-PCR from the spleen of a C57BL/6 mouse. Webspleen cells. After fusion of EL4/Mar (genotype H-2Kb, H-2Db, B2mb) with BALB.B (genotype H-2Kb, H-2Db, B2ma) spleen cells, 12 hybrids that grew in HAT medium were iso-lated and their surface phenotypes were determined by mi-crocytotoxicity testing. All of these hybrids expressed both H-2Kb and /32mb (Table 1). In these hybrids, the B2mb gene small wooden clothes horse
[Preparation of H-2Db tetramer and its application in ... - PubMed
WebDynamic expression of H-2Kb/Db in mouse hippocampus. (A) At P15, MHCI protein signals (green) were overlapped with those of NeuN (red, a marker for neurons) but not with GFAP (red, a marker for astrocytes). Scale bar: 50 μm. (B) The mRNA of H-2Kb and H-2Db was dynamically expressed in mouse hippocampus. Data was shown as ratio compared with … Web(H-2Db) Clone 28-14-8 MHC Class I (H-2Kb) Clone AF6-88.5.5.3 MHC Class I (H-2Kd) Clone SF1-1.1.1 MHC Class I (H-2Kd/H-2Dd) Clone 34-1-2S MHC Class I (H-2Kk) Clone … WebH-2Kb/H-2Db Antibody PE Applications: Flow Cytometry (FCM) Reactivity: Mouse (Ms) Conjugate/Tag: PE Quantity: 50 µg Citations: (2) Supplier Page Sign In or Register to view pricing Compare Product + See More (4) View All (22) MyBioSource.com Mouse Anti-MHC Class 1, H-2Kb Antibody Applications: Flow Cytometry (FCM) Reactivity: Mouse (Ms) small wooden chest box