2024 Halpern liver zonation

Halpern liver zonation

Author: oanm

August undefined, 2024

WebNational Center for Biotechnology Information WebMay 4, 2024 · It has been shown by single-molecule fluorescence in situ hybridization (smFISH) that the liver is divided into 9 lobular layers from the central to the portal veins, while single-cell RNA sequencing showed that the hepatic enzymes required for biosynthesis of bile acid are collaterally expressed from the central to the portal veins …

Single-cell technologies in hepatology: new insights …

WebJun 1, 2024 · In addition to providing new insights into liver endothelial cell heterogeneity, scRNAseq has revealed previously unknown functional zonation of LSECs across the liver lobule. Halpern et al ... WebDec 17, 2024 · Spatially resolved single cell transcriptomics in mice revealed extensive zonation of hepatocyte gene expression along the lobule radial axis (Halpern et al, 2024), and pseudotime analysis of scRNA-seq data suggested that similar zonation may also be prominent in the human liver (Aizarani et al, 2024). list of all gender identities

Spatial sorting enables comprehensive characterization of liver zonation

WebAug 22, 2024 · The liver metabolizes a variety of substances that sometimes interact and regulate each other. The modeling of a single cell or a single metabolic pathway does not represent the complexity of the organ, including metabolic zonation (heterogeneity of functions) along with liver sinusoids. Here, we integrated multiple metabolic pathways … WebJun 15, 2016 · The non-monotonic spatial zonation profile of liver polyploidy uncovered here differs from all previously documented liver zonation patterns, such as those of gluconeogenesis, urea formation, glutamine synthesis, ... Bahar Halpern K, Tanami S, Landen S et al (2015) Bursty Gene Expression in the Intact Mammalian Liver. WebFeb 16, 2024 · Abstract. The mammalian liver consists of hexagon-shaped lobules that are radially polarized by blood flow and morphogens. Key liver genes have been … images of house interior

Single-cell technologies in hepatology: new insights …

Midlobular zone 2 hepatocytes: A gatekeeper of liver …

WebJun 23, 2024 · Liver zonation is fundamental to normal liver function, and numerous studies have investigated the microstructure of normal liver lobules. However, only a few studies have explored the zonation signature in hepatocellular carcinoma (HCC). ... numerous studies have researched the micro-anatomical structure of the normal liver … Web(A) Model of a pentagon hepatic nodule with portal nodes (PN) surrounding the central vein (CV) in a zonal pattern extrapolated on the findings described by Bahar Halpern et al. 2 (B) The hepatocyte plate between the central vein and portal node was divided into nine regions based on expression of six different genes, and transcriptomes quantified. (C) Traditional … images of household objectsWebMar 2, 2024 · Halpern et al. and Moshe et al. combined single-molecule fluorescence in situ hybridization (smFISH) or fluorescence-activated cell sorting (FACS) of liver zonation surface markers with scRNA-seq to reveal the zonation patterns of hepatocyte gene expression (14, 15). Genes that were overexpressed in the PN area in both Halpern et … images of household bugs

"WebThis is made possible by the spatial compartmentalization of metabolic pathways a phenomenon known as liver zonation. Despite the importance of zonation to normal liver function, it is unresolved if and how … " - Halpern liver zonation

Halpern liver zonation

Single-cell spatial reconstruction reveals global division of labour in the mammalian liver

WebVanderbilt Orthopaedics. 1215 21st Avenue South, Suites 3200 and 4200. Nashville, TN 37232. Make an Appointment. (615) 936-7846. Get Directions. WebSep 8, 2024 · The liver is organized in polygonal lobules. Blood flows from the portal venuole and hepatic arteriole through sinusoids to the central vein. Fourteen to twenty …

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WebJul 14, 2014 · Abstract. Liver zonation, the spatial separation of the immense spectrum of different metabolic pathways along the liver sinusoids, is fundamental for proper functioning of this organ. Recent progress in elucidating localization and interactions of different metabolic pathways by using "omics" techniques and novel approaches to couple them … WebNov 12, 2024 · Zonation across the homeostatic liver lobule has recently been characterized in hepatocytes and endothelial cells using scRNA-seq approaches …

WebThis phenomenon is termed liver zonation 1,7. Recently, Halpern et al. combined single-cell RNA-sequencing (scRNA-seq) of dissociated hepatocytes and single-molecule RNA fluorescence in situ ... WebFeb 17, 2024 · The concept of hepatocyte functional zonation is well established, with differences in metabolism and xenobiotic processing determined by multiple factors including oxygen and nutrient levels across the hepatic lobule. However, recent advances in single-cell genomics technologies, including single-cell and nuclei RNA sequencing, and …

WebFeb 17, 2024 · Here we discuss how these transformative experimental strategies are being leveraged to dissect liver zonation at unprecedented resolution and how this new information should facilitate the emergence of novel precision medicine-based therapies for patients with liver disease. WebJan 26, 2024 · The mammalian liver is composed of repeating hexagonal units termed lobules. Spatially-resolved single-cell transcriptomics revealed that about half of hepatocyte genes are differentially expressed across the lobule. Technical limitations impede reconstructing similar global spatial maps of other hepatocyte features. Here, we used …

WebDr. Halperin is currently a Professor of Medicine, Radiology and Biomedical Engineering at the Johns Hopkins Medical Institutions. He is the co-director of the …

images of house plantsWebFeb 17, 2024 · INTRODUCTION. The concept of hepatocyte functional zonation has been known for some time, with differences in metabolism and xenobiotic processing determined by multiple factors including … list of all geoganWebJan 11, 2024 · Despite the concerted regulation of liver function by these two regulatory mechanisms, combined assessment of both zonation and chronobiology in the liver at single-cell resolution has been lacking. list of all genders and sexualitiesWebJun 23, 2024 · We found that liver zonation-related genes are liver-specific and commonly downregulated in HCCs. The liver zonation-related signature (LZRS) is a reliable … list of all genesWebUsing the spatial information from hepatocytes, we reconstructed LEC zonation and extracted a landmark gene panel that we used to spatially map LEC scRNAseq data. … list of all genetic disordersWebOct 8, 2024 · Halpern, K. B. et al. Single-cell spatial reconstruction reveals global division of labour in the mammalian liver. ... Gebhardt, R. & Matz … images of house plansWebFeb 7, 2024 · Combined use of scRNA-seq with single-molecule fluorescence in situ hybridization allowed reconstruction of liver zonation and inference of lobule coordinates in fasted adult mice, with more than half of the liver genes shown to be significantly zonated, albeit the analysis was focused on hepatocytes only (Halpern et al., 2024). images of house numbers on houses