Hybond n+ filter
WebUse for the highest sensitivity in DNA and RNA blotting. Cytiva Life Sciences™ Amersham™ Hybond™ -N+ Membrane is a positively charged nylon membranes for use with … Web31 mei 2024 · The hybridization signal was detected by the Gene Images CDP-Star detection module (Amersham Pharmacia Biotech, Piscataway, NJ, USA) after washing at a stringency of 0.2 × SSC, 0.1% SDS for 2 × 50 min. The filters were exposed to X-ray films for signal detection. 3. Results 3.1.
Hybond n+ filter
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http://novelfull.to/search-ilmsy/139mm-H-Straight-Shank-265558/ WebNylon membranes for Southern and northern blotting. Charged nylon membranes for nucleic acid blotting
WebF. oxysporum (Fol4287 and Bt.01) and C. higginsianum (IMI349063A) conidiospores were transformed on a Hybond-N+ filter by A. tumefaciens carrying the pRF-HU2 plasmid … WebAdvancing Eucalyptus genomics: identification and sequencing of lignin biosynthesis genes from deep-coverage BAC libraries
Webted onto HybondTM-N+ membrane (Amersham Phar-macia Biotech) by capillary transfer. The DNA was cross-linked to the membrane by UV irradiation. South-ern hybridization was performed according to “The DIG System User’s Guide for Filter Hybridization” (Boehringer Mannhein). DIG-labeled tctrx gene was used as probe for hybridization assay.
WebHybond-N+ filters (Amersham) (10). Hybridization. (i) Oligonucleotide probes. Oligonucleotide 1818 was labeled with 32P at its 5' end with phage T4 polynucleotide …
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