WebWestern blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG … WebWhen I did the Western blot analysis of PARP protein (113 kDa and 89 kDa) (which is an indicator of apoptosis), the untreated cells showed the band of cleaved PARP (89kDa).
Role of ERβ palmitoylation in the inhibition of human colon cancer …
WebTo further evaluate the role of cytoplasmic p21 in 5FU resistance, control as well as separately collected resistant (R, adherent) and dead (D, detached) cells after 5FU exposure for 48 h were analyzed for PARP cleavage, p21, and p-p21 T145 protein expression by Western Blot analysis. PARP is known to be one of the major targets of caspase-3 ... WebPoly(ADP-ribose) polymerase (PARP) is a substrate for CPP32/caspase-3, a member of the ICE family of proteases. The PARP monoclonal antibody can be used to detect PARP cleavage by western blot analysis, providing a useful marker for early apoptosis. sunnyvale parks and recreation department
JCM Free Full-Text SET Overexpression is Associated with …
Web16 Oct 2015 · To assess the reliance of the cervical cancer cells on Bcl-2 in the presence of cisplatin, Bcl-2 knock-down was achieved through RNA interference, where after apoptosis was assessed through PARP cleavage (Western blotting), Caspase activity (Caspase-Glo ©) and PI inclusion analysis (Flow cytometry). Finally, pre-malignant and malignant cervical … Web15 May 2024 · Representative Western blots show an increase in p66Shc activity (A), γH2AX phosphorylation (B), and PARP cleavage (C) in MCF-7, PC3, and DU-145 cells stimulated with 50 μM Rigosertib for 18 h. Numbers on top of the blot indicate the fold change in protein phosphorylation upon Rigosertib treatment (normalized to loading control) relative to … WebSpecificity. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP1 and can be used as marker for detecting apoptotic cells. Cleavage site specific antibody, … sunnyvale property tax rate