2024 Specific-locus amplified fragment sequencing

Specific-locus amplified fragment sequencing

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August undefined, 2024

WebApr 8, 2024 · Every single fragment individually amplified with newly designed primers was verified by sequencing as well as by fragment analysis. The specificity of primers was tested using cultivars with different allele compositions to exclude false positivity. In all cases, primers proved to be 100% specific. WebSep 2, 2016 · Recently, a more effective method called specific-locus amplified fragment sequencing (SLAF-seq) was proposed by Sun et al. 24. SLAF-seq is used a pre-designed reduced representation strategy and ...

Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Webfour RAPD fragments amplified from genomic loci. We have used a set of decamer oligonucleotides that extend the nonamer sequence by a single base at the 3'-terminus (A, T, C or G), as independent sequencing primers for each RAPD strand. Employing cycle-sequencing dye-terminator chemistry and automated four colour sequencing, each strand … WebResume But de l’etude Caracterisation phenotypique et genotypique de 96 isolats cliniques de Pseudomonas aeruginosa dans un hopital universitaire tunisien sur une periode de 16 mois. Materiels et methodes Tous les isolats ont ete caracterises par serotypie et antibiotypie, puis genotypes par deux techniques : randomly amplified polymorphic DNA … christopher columbus brought what diseases

SLAF-Seq - Illumina, Inc.

WebSpecific length amplified fragment sequencing (SLAF-seq) technology is a simplified genome sequencing technology based on next-generation sequencing. SLAF-seq … WebJul 31, 2024 · By combining next-generation sequencing (NGS) technology with reduced representation libraries (RRLs), specific-locus amplified fragment sequencing (SLAF-seq) enables large-scale SNP discovery and genotyping of mapping populations to generate high-density linkage maps in a low-cost but efficient manner [ 25, 26 ]. WebNov 21, 2024 · Construction of a high-density genetic linkage map and QTL mapping of oleic acid content and three agronomic traits in sunflower (Helianthus annuu L.) using Specific-locus amplified fragment... getting from thessaloniki to kavala

Large-Scale SNP Discovery and Genotyping for Constructing a …

WebDec 28, 2024 · In total, 115.95 M reads, 56,355 polymorphic specific-locus amplified fragments were developed, and 32,556 reliable single nucleotides (SNPs) were detected. The results indicated that the genotypes of many polymorphism sites had low heterozygosity in every population. WebAug 2, 2015 · Specific - locus amplified fragment sequencing (SLAF - seq), a recently developed strategy for large scale de novo discovery and genotyping of single nucleotide polymorphisms (SNPs), was employed in this study to develop sufficient markers for a segregating Agropyron F 1 population derived from an interspecific cross between two … getting from terminal 7 to terminal b at laxWebMay 7, 2024 · A high-density genetic map developed by specific-locus amplified fragment (SLAF) sequencing and identification of a locus controlling anthocyanin pigmentation in … getting from tromso airport to city

"WebJun 2, 2015 · We developed a total of 6,042 valid SNP markers using specific-locus amplified fragment sequencing (SLAF-seq), and subsequently mapped them into the previous framework map. The final map contained 6,448 molecular markers, distributing on fifteen linkage groups corresponding to the number of tea plant chromosomes. " - Specific-locus amplified fragment sequencing

Specific-locus amplified fragment sequencing

WebJul 22, 2024 · Specific locus amplified fragment sequencing (SLAF-seq) technology was used to construct a genetic map that covered 3353.15 cM with an average distance between consecutive markers of 0.48 cM. The seed index, together with kernel size, oil and protein content were further used to identify QTL. WebFeb 10, 2024 · The amplified fragments were visualized with ethidium bromide in a 1.5% agarose gel. Yeast two-hybrid assay The pGADT7 vector, which contains the GAL4 …

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WebFeb 10, 2024 · The F 2 population and the specific-locus amplified fragment sequencing (SLAF-seq) technique were used to construct a high-density genetic map (1230.16 cM), which contained 4032 markers distributed in nine linkage groups, with a mean distance between markers of 0.31 cM. WebJan 18, 2007 · SSAP is a 'multiplex' approach, which produces multiple markers per experiment and is therefore comparable to similar marker methods — such as amplified …

WebSpecific locus amplified fragment sequencing (SLAF-seq) is an optimized version of ddRADseq, specifically intended for large-scale genotyping experiments. The enzymes … WebMar 29, 2024 · To examine the genetic architecture of cotton VW resistance, we performed a genome-wide association study (GWAS) using a panel of 299 accessions and 85 630 single nucleotide polymorphisms (SNPs) detected using the specific-locus amplified fragment sequencing (SLAF-seq) approach.

WebMay 26, 2015 · Specific-locus amplified fragment sequencing (SLAF-seq) is a recent molecular marker development technology that enable the large-scale discovery and … WebFeb 14, 2024 · Specific-locus amplified fragment sequencing (SLAF-seq) is a high-throughput technique widely adopted for the development of SNP markers in different species, such as the genetic map of peaches spanning 1098.79 cM with an average distance of 0.17 cM between adjacent markers ( Shi et al., 2024) and the map of tea covering a …

WebHerein, we used specific-locus amplified fragment sequencing to generate a genetic delineation of P. cablin collected from Vietnam, South China, and Indonesia (Sumatra). In …

WebThis is commonly achieved by extracting restriction fragment within given size range, which is named reduced representation library (RRL). Specific-locus amplified fragment … getting from tokyo to sapporoWebBriefly, a 157-bp fragment of the Ki-ras gene was amplified with 0.5 μmol/L each of the ... success of WGA and subsequent locus-specific PCRs requires quick handling of fresh or thawed tissues, especially during microdissection, to avoid DNA degradation by nucleases. ... Furthermore, sequencing of specific DNA fragments after preamplification ... getting from treviso airport to veniceWebJun 2, 2015 · DNA fragments from 400 to 450 bp (SLAFs) were excised and isolated using a Gel Extraction Kit (Qiagen), and were then subjected to PCR amplification with Phusion … christopher columbus born cityWebSep 30, 2009 · In this comparative genomic analysis, multi-locus sequence typing (MLST), amplified fragment length polymorphism (AFLP), LOS class PCR typing, and pulsed-field gel electrophoresis (PFGE) were employed to define detailed strain specific genotypes. ... (A-E) screened by the class-specific PCR. The class A LOS locus was significantly associated ... christopher columbus bugs bunnyWebMar 30, 2024 · Specific-Locus Amplified Fragment Library Construction for High-Throughput Sequencing Specific length amplified fragment sequencing (SLAF-seq) is an efficient method of large-scale genotyping developed on the basis of high-throughput sequencing technology and reduced representation library (RRL). getting from trieste to venice christopher columbus burial placeWebJul 1, 2024 · Using specific-locus amplified fragment sequencing (SLAF-seq) technology, we constructed a high-density genetic linkage map with an average map distance of 0.76 cM. Three major QTLs controlling... getting from thailand to cambodia